Figure 4

Shared polymorphisms in species from the Balkans. * Two accessions of this species are identical for these characters (except for H. gymnocephalum 1215 with C instead of Y at position 344); ¹ Interclade hybrids; ² The 'unknown Western 2' polymorphisms (light violet) were not found in direct sequencing, but are represented by a single cloned sequence and are probably present in very low amounts in this genome. Dark orange: apomorphic substitutions of Balkan species; light orange: shared substitutions of Balkan species and hybrids; green: polymorphisms shared only by Balkan interclade hybrids, but not reflected by 'pure' Balkan species ('unknown Eastern'); light violet: polymorphisms shared with the (non-Balkan) interclade hybrid accessions H. villosum 1305 and H. pilosum 1226/2, but not reflected by other Balkan species ('unknown Western 2'). At position 254, C occurs on the 'unknown Western 2' ribotype in H. plumulosum and H. gymnocephalum, but on the 'Eastern' ribotype in H. olympicum and must therefore be a parallelism. The scattered and inconclusive distribution of polymorphisms and substitutions in 'pure' Balkan species are reflected by the intra-individual patterns in cloned sequences of H. kittanae, their most polymorphic representative. For more details, see Additional file 2: Patterns of ETS recombination.