Figure 4

Expression of goat V1R genes in the VNO and the MOE. A: Expression of goat V1R genes in the VNO and the MOE was examined by nested RT-PCR. The cDNAs were synthesized with (+) or without (-) reverse transcriptase. Specific primers for detecting each V1R gene are listed in Additional file 4. B: Summary of the expression of goat V1R genes detected by nested RT-PCR analysis. All goat V1Rs identified thus far are expressed in the VNO and in the MOE (indicated by +). C: In situ hybridization analysis. Coronal sections from goat VNO or MOE were hybridized with antisense V1R probes. Arrowheads indicate V1R-expressing cells. "n" indicates the neuroepithelial layer. Scale bar indicates 50 μm.