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Table 4 Primers used for the quantification of fabp mRNA and hnRNA

From: Differential transcriptional modulation of duplicated fatty acid-binding protein genes by dietary fatty acids in zebrafish (Danio rerio): evidence for subfunctionalization or neofunctionalization of duplicated genes

Gene

Forward primer

Reverse primer

  
 

mRNA quantification

AT 1

Entrez gene ID

fabp1a

TAAGCTGACAGCGTTTGTGAAGGG

AGATGCGTCTGCTGATCCTCTTGT

60.0

791610

fabp1b.1

AAGCTGAAGGTGGTGCTGAACA

CACGTTTGCTGATGCGCTTGTA

58.0

554095

fabp1b.2

TGCCGTTCTCTGGGAAGTTTGAGT

TGACTTTGTCTCCGCTCAGCATCT

67.5

 

fabp7a

TGTGCCACTTGGAAACTGGTTGAC

AACATTGCCTACTTGCCTGGTAGG

56.5

58128

fabp7b

AAACCACTGCTGATGACCGACACT

AGTGGTCTCTTTCCCATCCCACTT

56.0

407736

fabp11a

TGTGCAGAAACAGACCTGGGA

ACAGCCACCACATCACCCATCTT

56.0

447944

fabp11b

GCTGTCACTACATTCAAGACCTG

AGTTTACCATCCGCAAGGCTCA

56.0

553579

ef1α

TTGAGAAGAAAATCGGTGGTGCTG

GGAACGGTGTGATTGAGGGAAATTC

59.6

30516

 

hnRNA quantification

  

fabp1b.1

GAACTAACGTGTGCTGCTTGTG2

CACGTTTGCTGATGCGCTTGTA3

60.0

554095

fabp7a

CCATCCATCAGATTTCTATGTGGG2

CATTATGCCTTCTCGTATGTGCG3

58.0

58128

fabp7b

TTGGAAATGTGACCAAACCGACGC3

TCGTCTCGAAAGGGAATGCAGTGT2

59.0

407736

fabp11a

CCAAGCCGTTTTTGATGATGTGAG2

GCTATTAATTTCCCATCCGACACC3

61.0

447944

ef1α

AGCCTTGCATTCGTGCTGAAGT2

GGAACGGTGTGATTGAGGGAAATTC3

59.0

30516

  1. 1 AT, annealing temperature
  2. Primer based on intronic2 and exonic3 sequences for gene-specific amplification.