Evolutionary rate patterns of the Gibberellin pathway genes

Table 1 Information on the genes and sequences of the primers used in the study.

Gene	Location^a	Enzyme	Aligned coding length (bp)	GC (GC3)^b	ENC^b	Primer sequences^c
CPS1	2	ent-copalyl diphosphate synthases I	1002	0.462 (0.494)	54.6	F1: AACTTGTGGAGGTTAGCAG F2: TGTGGAGGYTAGCRGAGG R1: AAGTCGCTCAGAGGCACG R2: TAGCCCATGCAAGTCGCTC
KS1	4	ent-kaurene synthase I	1023	0.426 (0.417)	52.8	F1: TGCTGAAGCTTCCAGTTTCC F2: TCCAGTTTCCGTGAATCAC R1: CTTGCACATCTTCCAGAAC R2: CCTTGACGACTGCATTCAC
KO2	6	ent-kaurene oxidase II	1050	0.514 (0.639)	58.2	F: CTGTAGTTGTGCTCAATTC R1: GCCATCGTCTTGTACATGTC R2: TCAGCCTCCACYCGAACTC
KAO	6	ent-kaurenoic acid oxidase	1053	0.603 (0.820)	39.2	F: CAGGACGTTCATGTTCAGCAG R1: TCGTCGCCAAGCAGTTGTC R2: GCCAAGCAGTTGTCCAC
GA20ox2	1	GA20-oxidase II	597	0.714 (0.966)	30.1	F: ATCCCGGAGCCATTCGTSTG R: TGAAGGTGTCGCCGATGTTG
GA3ox2	1	GA3-oxidase II	786	0.710 (0.965)	30.4	F1: ACCCGCTCTRCTTCGACTTC F2: GGCGGGTGCCGGAGACGCACG R: CCATGTACTCSGGCCACGTGAC
GA2ox4	5	GA2-oxidase IV	819	0.694 (0.926)	34.0	F: GAGCAGATCTCGCTGSTGAG R1: CAGGCGGTTGTCGCYGAG R2: AGGCGGGAGAGGTAGGCAG
Total			6330

^aChromosomal locations in O. sativa.
^bAverage of 8 Oryzeae species.
^cLocations of the primers for each fragment are showed in Additional file 2. The internal primers were used for cycle sequencing are listed in Additional file 5.

ISSN: 2730-7182