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Table 2 Frequencies of 38Ser- and 38Gly-coding KCNE1 alleles (%112A/%112G) in gDNAs and cDNAs of brain samples (frontal cortex), as inferred by pyrosequencing

From: Positive selection at codon 38 of the human KCNE1 (= minK) gene and sporadic absence of 38Ser-coding mRNAs in Gly38Ser heterozygotes

Brain

sample

gDNA (%112A/%112G)

cDNA (%112A/%112G)

  

1st Run

2nd Run

1

0.0/100.0

-

-

2

0.0/100.0

-

-

3

0.0/100.0

-

-

4

100.0/0.0

100.0/0.0

100.0/0.0

5

51.8/48.2

60.0/40.0

50.1/49.9

6

0.0/100.0

-

-

7

0.0/100.0

-

-

8

52.5/47.5

52.6/47.4

53.6/46.4

9

100.0/0.0

100.0/0.0

100.0/0.0

10

100.0/0.0

100.0/0.0

100.0/0.0

11

22.2/77.8*

0.0/100.0

0.0/100.0

12

61.8/38.2

48.1/51.9

56.4/43.6

13

100.0/0.0

100.0/0.0

100.0/0.0

14

54.5/45.5

59.6/40.4

60.0/40.0

  1. *The genotype of brain sample 11 has been verified by a total of eight parallel measurements (not shown). It is marked with bold as it exerts an absolute depletion of 38Ser-coding alleles (112A) at the cDNA level.
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BMC Ecology and Evolution

ISSN: 2730-7182

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