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Table 1 Frequencies of 38Ser- and 38Gly-coding KCNE1 alleles (%112A/%112G) in gDNAs and cDNAs of lymphoblastoid cell lines, as inferred by pyrosequencing

From: Positive selection at codon 38 of the human KCNE1 (= minK) gene and sporadic absence of 38Ser-coding mRNAs in Gly38Ser heterozygotes

Lymphoblastoid

cell line

gDNA (%112A/%112G)

cDNA (%112A/%112G)

 

1st Run

2nd Run

1st Run

2nd Run

1

56.4/43.6

54.7/45.3

54.7/45.3

59.8/50.2

2

54.7/45.3

52.7/47.3

40.6/59.4

59.2/40.8

3

100.0/0.0

100.0/0.0

100.0/0.0

100.0/0.0

4

48.7/51.3

46.8/53.2

39.5/60.5

45.1/54.9

5

56.6/43.4

57.2/42.8

61.6/38.4

54.2/45.8

6

100.0/0.0

100.0/0.0

100.0/0.0

100.0/0.0

7

57.5/42.5

53.0/47.0

60.8/39.2

56.2/43.8

8

56.5/43.5

55.3/44.7

58.3/41.7

59.4/40.6

9

0.0/100.0

0.0/100.0

0.0/100.0

0.0/100.0

10

0.0/100.0

0.0/100.0

0.0/100.0

0.0/100.0

11

0.0/100.0

0.0/100.0

0.0/100.0

5.9/94.1

12

0.0/100.0

0.0/100.0

0.0/100.0

0.0/100.0

13

53.1/46.9

55.2/44.8

60.0/40.0

55.1/44.9

14

56.3/43.7

54.9/45.1

45.9/54.1

62.5/37.5

15

56.7/43.3

55.7/44.3

59.3/40.7

69.2/30.8

16

56.0/44.0

54.9/45.1

59.3/40.7

53.1/46.9

17

57.6/42.4

51.7/48.3

63.6/36.4

56.5/43.5

18

54.7/45.3

50.1/49.9

60.0/40.0

54.6/45.4

19

52.9/47.1

56.5/43.5

51.2/48.8

51.5/48.5

20

54.4/45.6

52.8/47.2

52.7/47.3

51.4/48.6

21

100.0/0.0

100.0/0.0

96.6/3.4

100.0/0.0

22

100.0/0.0

100.0/0.0

100.0/0.0

100.0/0.0

23

56.2/43.8

56.0/44.0

62.6/37.4

52.2/47.8

24

55.0/45.0

53.2/46.8

0.0/100.0

0.0/100.0

25

100.0/0.0

100.0/0.0

100.0/0.0

96.8/3.2

26

54.1/45.9

54.7/45.3

0.0/100.0

0.0/100.0

27

100.0/0.0

100.0/0.0

100.0/0.0

97.1/2.9

28

49.6/50.4

52.0/48.0

59.7/40.3

59.3/40.7

29

52.3/47.7

50.5/49.5

52.1/47.9

48.4/51.6

  1. Lymphoblastoid cell lines with an absolute depletion of 38Ser-coding alleles (112A) at the cDNA level are highlighted with bold.