Skip to main content

Table 1 Sea lamprey olfactory organ CR gene expression from 454, EST and RT-PCR data.

From: The sea lamprey Petromyzon marinusgenome reveals the early origin of several chemosensory receptor families in the vertebrate lineage

 

olfactory organ 454 transcripts

RT-PCR Results

      

Gene

(Adult, Parasite)

olfactory

brain

liver

gill

kidney

gonad

RT-primer 5'-3'

Tm

1681.OR230*

3,4

x

    

x

tcaataatggagtcgcttgc

60

        

tctcacgttgaaatgccaag

 

2061.V1R320

0,2

x

x

   

x

gcagtgtcgtggtctctcaa

56

        

gaatgtagcgcgagttctcc

 

18775.V1R342

3,1

x

x

x

x

x

x

tctgataagcctcgcgttct

54

        

ctcgggcacatttggtattt

 

6425.OR330 a

1,5

x

    

x

ccgtactgcgactacctcgt

58

        

ggcactcgtagaggatgagc

 

2407.OR326

2,0

x

x

x

  

x

gctcgaaggatttcaacagg

57

        

aggtcacggtcctcacaaag

 

3267.OR325

7,1

x

x

   

x

gtagtggctttgggcatcat

57

        

cacgaatattgccacgaaaa

 

3721.TAAR351

2,0

x

    

x

gtgtggaccttccaccaagt

60

        

gcagctgcctgaagtagagg

 

9755.TAAR355 a

2,0

x

    

x

gtagccatcgccttcttcag

60

        

gaggcgtagaaccagcactc

 

16230.TAAR353

0,1

x

     

ctgcgtcgactccttctacc

52

        

ggaggagttcgtcagcatgt

 

3717.V1R311

not detected

x

    

x

tgaagaatggggaactgctc

54

        

atacaagagcaaccggcatc

 

1548.PRH340

not detected

 

x

x

  

x

aacgtgaccaacctgctcat

54

        

gctgcatgagaaacacgaag

 

11722.V1R311

not detected

x

     

atcaagacgctgctcatcct

54

        

cccagcagatcacgaatatg

 

5673.OR343

not detected

 

x

    

atcctcctgtgcaacctgtc

60

        

ggccggcagatgtagaagta

 

2594.TAAR358

not detected

x

     

tatctcttggctgccgttct

50

        

gccggagacaaaaacacatt

 

107483.OR345

not detected

x

     

tccacatccagatggtgttc

60

        

ttggtgttctccaccaggtc

 

17613.OR230

not detected,

      

atcatggtcacctcctacgc

60

 

pseudogene

      

ggcaaccagaagatcaggaa

 

9755.TAAR355 b

2,0

      

no RT primers

 

14718.TAAR353

1,0

      

no RT primers

 

2008.CASR839

1,1

      

no RT primers

 

10424.MGR935

1,1

      

no RT primers

 

3267.OR361

0,1

      

no RT primers

 

1268.OR328**

0,0

 

NCBI

ESTs

from

embryo

 

EG337313

 
        

EG335699

 

10796.OR320

2,2

      

no RT primers

 

12707.TAAR348

2,0

      

no RT primers

 

7812.OR322

1,0

      

no RT primers

 

14563.OR381

0,1

      

no RT primers

 

15806.TAP†

1,0

      

no RT primers

 
  1. Expression data is shown for a subset of lamprey CR genes, including all OR, TAAR and V1R genes detected by olfactory organ cDNA 454 sequencing. RT-PCR results, primer sequences, annealing temperatures and NCBI EST data are also included. Gonad includes both adult testis and undifferentiated gonad from parasites. The partial gene 1681.OR230 corresponds to three ESTs (EE739806, EE738866 and EB081557) from adult olfactory organ*. Two sequences from the NCBI EST database (EG337313 and EG335699) obtained from sea lamprey embryo map to the gene 1268.OR328**. Expression of a calcium-sensing receptor (2008.CASR839) and a metabotropic glutamate receptor (10424.MGR935) were detected in the olfactory organs of adult sea lamprey. A 540 bp EST represents a cellular nucleic acid binding protein† expressed in both adult olfactory organ and embryo that was not included in the phylogenetic analysis.
Back to article page

BMC Ecology and Evolution

ISSN: 2730-7182

Contact us