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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Zebrafish RNase T2 genes and the evolution of secretory ribonucleases in animals

Figure 2

The zebrafish genome contains two RNase T2 genes. A) Alignment of the two predicted RNase T2 proteins (RNase Dre1 and RNase Dre2) present in the zebrafish genome with RNase T2 proteins from salmon (RNase Ok2), human (RNASET2), Arabidopsis thaliana (RNS1), tomato (RNase LE) and Aspergillus oryzae (RNase T2). Residues conserved in all RNase T2 enzymes are highlighted. CAS I and CAS II, conserved active-site segments that contain the two Histidines (*) involved in catalysis. The predicted signal peptides for RNase Dre1 and RNase DRe2 are underlined, and the alternative starting Methionine in RNase Dre1 is double-underlined. B) Structure of the two RNase T2 genes identified in the zebrafish genome. The intron-exon structure was obtained by comparison of the sequences obtained from direct cloning and sequencing of cDNA with the publish sequence of genomic DNA. Boxes indicate exons, lines indicate introns. Gray shading indicates untranslated regions, white indicates coding region, and black marks the region that undergoes alternative splicing in RNase Dre1. Gene accession numbers for the zebrafish proteins are FJ460212 for RNase Dre2 and FJ460210 and FJ460211 for the two different splicing variants of RNase Dre1.

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