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Figure 1 | BMC Evolutionary Biology

Figure 1

From: Zebrafish RNase T2 genes and the evolution of secretory ribonucleases in animals

Figure 1

Characterization of zebrafish RNases. A) Ribonuclease activities present in zebrafish extracts. Adult zebrafish extracts were analyzed in an in gel RNase assay at two different pHs. Adult zebrafish of mixed sexes were separated into "body" (B, mostly muscle, skin and skeleton), "head" (H, which included skull, muscle, skin, brain, eyes among other tissues) and "gut" (G, which included most internal organs such as gut, liver, sexual organs, heart). The size range for RNase T2 and RNase A proteins is indicated. B) Same samples as in A, analyzed by SDS-PAGE and stained with Coomassie Blue. One hundred μg of protein per lane were analyzed in both types of gels.

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