Figure 6

Ivermectin potentiates ATP evoked currents at Hd P2X. Two-electrode voltage clamp recordings were made from Xenopus oocytes expressing HdP2X. A. Representative ATP (100 μM) evoked currents recorded from the same cell in the presence and absence of 3 μM ivermectin (ATP/ivermectin application indicated by bars). Five minutes recovery was allowed between applications and ivermectin was bath perfused at 3 μM in the five minutes preceding the second recording as well as being present in the ATP application. B. Concentration response curves for ATP in the presence (open circles) and absence (closed squares) of 3 μM ivermectin (n = 7). Mean currents were normalised to the response produced by 100 μM ATP in the absence of ivermectin.