Figure 1

Amino acid alignment of Hd P2X. The predicted amino acid sequence of HdP2X was aligned with human P2X_{1, and 4}, rat P2X₂ and non vertebrate P2X channels from S. mansoni (smP2X) [12]O. tauri (OtP2X) [11], M. brevicollis (MbP2X) [11] and D. discoideum (dP2XA) [10]. Predicted transmembrane regions in HdP2X (TopPred algorithm [19]) are depicted by black horizontal lines. Functionally important amino acid residues using human P2X₁ residue numbering (indicated by *) are highlighted in bold above the sequence including positively charged lysine residues, FT and NFR motifs, conserved cysteines and a conserved consensus protein kinase C phosphorylation site. Point mutations made in HdP2X to investigate zinc and copper binding are indicated in bold below the sequence.