Skip to main content

Table 1 List of microorganisms and plasmids

From: Independent inactivation of arginine decarboxylase genes by nonsense and missense mutations led to pseudogene formation in Chlamydia trachomatisserovar L2 and D strains

Strain or plasmid

Description and partial genotype

Reference or source

Chlamydia pneumoniae

Kajaani 6

[52]

C. trachomatis L2/434

Lymphogranuloma venerum II serovar L2, strain 434

H. Caldwell

C. trachomatis D/UW-3

Trachoma serovar D, strain UW-3/Cx

P. Wyrick

Escherichia coli

  

   BL21(DE3)

Expression strain with T7 RNA polymerase gene

Novagen

   DEG0147

MG1655 ΔadiAYC::kan

[17]

   DH5α

General cloning host

Invitrogen

   XL1-Blue

General cloning host

Stratagene

Plasmids

  

   pBAD/HisA

Expression vector with PBAD promoter

Invitrogen

   pDFclone1

L2/434 CTL0627–0628 in pGEM-T

This work

   pDG379

Cpn aaxBC in pBAD/HisA

[17]

   pDG479

L2/434 CTL0627–0628 in pBAD/HisA

This work

   pDG491

L2/434 His10-CTL0627 in pET-19b

This work

   pDG543

pDG479 with X128W substitution

This work

   pDG558

D/UW-3 His6-CT373–374 in pBAD/HisA

This work

   pTG17

D/UW-3 His10-CT373 in pET-19b

This work

   pTG20

D/UW-3 His10-CT373 Arg115Gly in pET-19b

This work