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Figure 4 | BMC Evolutionary Biology

Figure 4

From: Independent inactivation of arginine decarboxylase genes by nonsense and missense mutations led to pseudogene formation in Chlamydia trachomatisserovar L2 and D strains

Figure 4

SDS-PAGE analysis of affinity-purified proteins shows the AaxB variant proteins self-cleaved in E. coli. Lane M contains polypeptide markers (Bio-Rad). Lane 1 contains the X128W variant of His6-AaxB from strain L2/434, including proenzyme (26 kDa expected, 26 kDa observed), α-subunit (16 kDa expected, 16 kDa observed) and β-subunit (10 kDa expected, 8 kDa observed). Lane 2 contains His10-AaxB from strain D/UW-3, including only proenzyme (24 kDa expected, 25 kDa observed). Lane 3 contains the R115G variant of His10AaxB from strain D/UW-3, including proenzyme (25 kDa expected, 25 kDa observed), α-subunit (16 kDa expected, 16 kDa observed) and β-subunit (9 kDa expected, 6 kDa observed). The gel was stained with silver, and the image was processed as described for Figure 2.

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