Figure 3

Laforin orthologs in invertebrates. A, An alignment of N. vectensis (Nc-) laforin and three B. floridae (Bf-) laforin with H. sapiens (Hs-) laforin. Highlighted residues and shapes representing predicted secondary structure are as in Fig. 2. The red line above the sequence delineates the CBM and the blue line delineates the DSP. Green arrows and ovals represent a match with the predicted secondary structure of Hs-laforin, black arrows and ovals are shared between Nv-laforin and Bf-laforin, and brown are unique for Hs-laforin. The β-sheets and α-helices of the DSP are numbered according to standard DSP nomenclature [11]. The two asterisks indicated residues necessary for carbohydrate binding. B, Percent similarity and identity of Bf- and Nv-laforin with Hs-laforin. C, Predicted intron-exon boundaries for the genes encoding Hs-, Bf-, and Nv-laforin. The coding region that encodes the CBM is highlighted in red, the DSP in blue, and the rest is in gray.