Conservation of laforin in N. caninum. A, An alignment of N. caninum (Nc-) laforin, with T. gondii (Tg-) laforin, and H. sapiens (Hs-) laforin. Residues boxed in red are invariant CBM20 residues as defined by the CBM20 family , and residues boxed in blue are part of the DSP catalytic site. Residues boxed in dark gray are identical. Accession numbers are listed in Additional File 8. The red line above the sequence delineates the CBM and the blue line delineates the DSP. The predicted secondary structure is represented by arrows (β-sheets) and ovals (α-helices). Green arrows and ovals represent a match with the predicted secondary structure of Hs-laforin, black arrows and ovals are shared between Nc-laforin and Tg-laforin, and brown are unique for Hs-laforin. The β-sheets and α-helices of the DSP are numbered according to standard DSP nomenclature . B, Percent similarity and identity of Nc-laforin with Hs- and Tg-laforin.