Domain architecture and orthologs of laforin and SEX4. A, Laforin contains a carbohydrate binding module-family 20 (CBM20) and a dual specificity phosphatase (DSP) domain. B, SEX4 is composed of a chloroplast targeting peptide (cTP), dual specificity phosphatase (DSP) domain, and a carbohydrate binding module-family 21 (CBM21). C, Unrooted phylogeny of the small subunit ribosomal RNA (SSU rRNA) sequences was generated as described in Methods, and accession numbers are listed in Additional File 5. Organisms containing laforin are boxed in yellow and those containing SEX4 are boxed in green. Organisms in red lettering are the organisms identified in this study that contain a laforin ortholog. Alveolates are shaded with a gray background and vertebrates with a brown background. Bootstrap values are indicated by color coding as indicated in the inset.