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Table 1 The variation of amino acids caused by the nucleotide changes in genes. The SNPs were detected by primers at the position with bold numbers in the Site column.

From: Molecular evolution of dimeric α-amylase inhibitor genes in wild emmer wheat and its ecological association

Number

Site

Substitution

Amino acid position

Amino acid variation

Numbers of gene sample

1

5

T-G-A

Signal peptide

Leu-Arg-His

241-1-2

2

19

G-A

Signal peptide

Val-Asp

59–185

3

25

G-T

Signal peptide

Ala-Ser

154-90

4

28

A-G-C

Signal peptide

Lys-Glu-Gln

59-170-15

5

31

T-C

Signal peptide

Tyr-His

242-2

6

34/35

G/A-A/A-G/G-A/G

Signal peptide

Asp-Asn-Gly-Ser

139-11-91-3

7

46/47

G/A-G/T-A/T-T/A-G/G

Signal peptide

Asp-Val-Ile-Tyr-Gly

57-74-7-91-15

8

79/81

T/T-C/G

10

Tyr-Gln

129-115

9

88/89

C/A-A/A-C/G

13

Gln-Lys-Arg

174-68-2

10

107

G-A-C

19

Gly-Asp-Ala

224-2-18

11

118

C-G-T

23

Leu-Val-Leu

62-66-116

12

125

A-G

25

Lys-Arg

219-25

13

127

C-G

26

Leu-Val

242-2

14

163

C-G

38

Leu-Val

180-64

15

190

A-G-C

47

Tyr-Asp-His

66-173-5

16

196

A-G

49

Ser-Gly

242-2

17

211

T-C

54

Cys-Arg

242-2

18

215/216

A/T-G/T-A/G-G/G

55

Asp-Gly-Glu-Gly

66-166-1-11

19

217

G-A

56

Ala-Thr

242-2

20

227

A-G

59

Asn-Ser

68–176

21

238/239

A/G-A/A-G/A

63

Ser-Asn-Asp

222-16-6

22

251

A-G

67

Glu-Gly

242-2

23

259/260

G/C-G/T-A/T

70

Ala-Val-Met

80-151-13

24

262/263

C/A-T/C-T/A

71

Gln-Ser-*

156-81-7

25

287

C-A

79

Ala-Glu

225-19

26

295

C-A

82

Thr-Lys

93–151

27

314

T-C

88

Val-Ala

241-3

28

320

T-C

90

Leu-Pro

242-2

29

343

G-A

98

Val-Ile

236-8

30

350

A-G

100

Lys-Arg

128-115

31

364

A-G

105

Ile-Val

129-114

32

380

G-A

110

Gly-Asp

72–172

33

382

A-G

111

Arg-Gly

70–174

34

391

A-G

114

Ile-Val

70–174

35

394

T-C

115

Cys-Arg

242-2

36

401

A-G-T

117

Asp-Gly-Val

183-60-1

37

409

A-G

120

Thr-Ala

68–176

38

416

G-A-C

122

Arg-Gln-Pro

68-15-161