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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Selection against tandem splice sites affecting structured protein regions

Figure 2

Avoidance of tandem acceptors in structured regions of Pfam domains. The distribution of exon/exon junctions derived from control introns, introns with tandem donors and acceptors (A) in alpha-helices, beta-sheets, and non-regular elements, (B) 'inside' or 'outside' structural elements (see text), (C) with respect to the average surface accessibility, and (D) with respect to the average inverse hydropathy scores. Kyte-Doolittle values were used to compute hydropathy scores for the ± 5 amino acid contexts. The values were inverted so that positive values indicate polar residues. To avoid potential biases, we excluded the insertion sequence of tandem donors and acceptors from the context. Different context lengths of ± 3, ± 10, or ± 15 residues give consistent results in D (Additional File 11). P-values using a χ2 test in A and B and a Wilcoxon rank sum test in C and D are indicated as *: P < 0.05, **: P < 0.001, ***: P < 0.0001.

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