Figure 1

Phylogenetic comparison of vertebrate NLR molecules. Amino acid sequences of the NACHT domains (between the GxxGxGKS/T motif and the 'FAAFY' signature of human NOD2 or equivalent region in other NLRs) of vertebrate NLRs were aligned using CLUSTALW. Trees were constructed from these multiple alignments using the Maximum evolution and Neighbor-joining methods within the MEGA 3.1 program, using Poisson correction and complete deletion of gaps. Maximum evolution trees are shown. The resulting trees were bootstrapped 1000 times (shown as percentages). [A] Zebrafish NLRs were compared to human NLRs to estimate orthology. [B] The NALP subfamily was analyzed in more detail by comparing all zebrafish and Xenopus tropicalis predicted NALP-like molecules to human NALPs. [C] The NOD/NLR-A subfamilies of zebrafish, frog, chicken and humans were compared. DR = Danio rerio; XT = Xenopus tropicalis; GG = Gallus gallus; HS = Homo sapiens.