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Table 1 Sequences of oligonucleotides used for PCR

From: Primate-specific spliced PMCHL RNAs are non-protein coding in human and macaque tissues

forward primer

sequence

reverse primer

sequence

T°

gene

Sequencing

     

1–2

CTCAAGGTATTTTACTTTCAGCATCC

1–3

TGCAGAATTTTCACAAAGTTTAATGCAC

56°C

PMCH

3–4

GGCCATAGGGTGGTTTGG

3–19

TGAGTAGATAAAAGGACTGACTT

56°C

PMCHL

3–18

AAGTCAGTCCTTTTATCTACTCA

3–22

TAACCTTGCTTTCTTCCTTTCTATA

57°C

PMCHL

3–15

GCGTCAGTGTCCTAATGCAT

3–68

GAATTTCTGAGCTGTGTTGTGC

58°C

PMCHL

3–18

AAGTCAGTCCTTTTATCTACTCA

3–27

TGATAACGTGAAATCGTACCAT

54°C

PMCHL

3–20

ACTCCACGTCAAGACAGTTGCA

3–30

GATGGAGGTAAACCAAGGAGG

60°C

PMCHL

3–72

TTCTGGTTTTCACAGTAACTGATCT

3–142

GGGAAATCAGTGAGTGGAGTAGGAA

58°C

PMCHL

3–110

GCTGAAATCCTTCCTCAAGA

3–33

AGCTGATATCCTAGAAGTAG

53°C

PMCHL

PCR

     

3–9

CTGAGAATGGGGTTCAGGATAC

3–30

GATGGAGGTAAACCAAGGAGG

58°C

PMCHL

3–9

CTGAGAATGGGGTTCAGGATAC

3–353

TTTAACAATTGAACACATGTAATCATT

53°C

PMCHL

3–10

TGGGGATGAAGAAAACTCAGCTAA

3–353

TTTAACAATTGAACACATGTAATCATT

53°C

PMCHL

3–7

CAATGGGATTATGCTGTCACAA

3–30

GATGGAGGTAAACCAAGGAGG

58°C

PMCHL

3–8

AACATAATTTCTTAAATCATGG

3–27

TGATAACGTGAAATCGTACCAT

56°C

PMCHL

Probe 1

     

3–25

TGAGATGTAAAGAGACCACCTT

3–30

GATGGAGGTAAACCAAGGAGG

58°C

PMCHL

  1. Annealing temperature (T°) that was used for PCR and gene-specificity are indicated for each oligonucleotide pair.