Collagenase-like sequences within the flagellin hypervariable region. A) A multiple alignment of CBO0798 and collagenase-containing flagellins identified by PSI-BLAST. Vertical black bars in the alignment correspond to the collagenase-containing region identified by a PSI-BLAST search using C. haemolyticum FliA(H) as the query. B) A schematic of a representative collagenase-containing flagellin based on the FliA(H) sequence. An alignment of similar HEXXH-containing segments from BoNT/B, a microbial collagenase, and the collagenase-containing flagellins are shown below the schematic. Accession numbers are provided in the Methods.