Protein sequence similarity heat map surrounding the BoNT/A neurotoxin gene cluster. Sequence similarity scores, E-values and percentile ranks were calculated for all pairwise combinations of putative proteins encoded in the C. botulinum strain A genome. A) A heat map of the percentile ranks for pairwise alignments involving 100 genes surrounding the neurotoxin gene cluster (described in Methods). B) Similarity ranks and E-values (in brackets) for pairwise protein sequence alignments in the neurotoxin gene cluster, corresponding to BoNT/A, NTNH, CBO0798, associated hemagglutinin components and other neighboring genes. E-values < 1 are in boldface.