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Table 1 Primers used in this study.

From: Transfer of rice mitochondrial ribosomal protein L6 gene to the nucleus: acquisition of the 5'-untranslated region via a transposable element

Primer Sequence
P1 5'-CCTCTTCTCTTGTTGccATGGAG-3'
P2 5'-CAGTACAGCAAATTAccaTGGGTTTG-3'
P3 5'-TTTCCCTGTCTTATTGCcATGGAA-3'
P4 5'-CCAGTACAGCATATTAccaTGGGTTTA-3'
P5 5'-CTGCTTCAAACtgtACaTAATTTGC-3'
P6 5'-GAAAGTAAAATgcGGccGCTCATCC-3'
P7 5'-TTGTTTCTGAAACTTGGTTACAG-3'
P8 5'-TTACTTCTTTTGCTTCTTCCCTGGCTT-3'
P9 5'-AATGG(A/C)AC(C/T)G(A/G)(A/T)ATGGTCAAG-3'
P10 5'-TTAGAAGCA(C/T)TTC(A/C)TGTG(C/G)AC-3'
  1. Underline: restriction sites for BsrGI, NcoI and NotI.
  2. Lowercase letters: deviations from the original OsRpl6-1 sequence to enable introduction of restriction sites.
  3. The locations of primers P1-P8 are shown in Figure 1A.
  4. Primers P9 and P10 were designed from rice Actin sequences [39] as described previously [40] with slight modifications.