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Table 1 Natural selection analysis. The mean of p values for all the comparisons among all yaaW and htgA homologs plus standard deviations, SD. * p < 0.05; ** p < 0.01. Neutrality: a statistically significant p value indicates non-neutral evolution; Purifying selection: a statistically significant p value indicates purifying selection

From: The origin of a novel gene through overprinting in Escherichia coli

  Neutrality Purifying selection
yaaW (all sequences) 0.042 ± 0.1215 * 0.024 ± 0.0869 *
A versus B 2.079E-09 ± 1.6218E-09 ** 1.168E-09 ± 6.5436E-10 **
A versus O 0.009 ± 0.0266 ** 0.005 ± 0.0139 **
B versus O 0.031 ± 0.0696 * 0.015 ± 0.0337 *
htgA (all sequences) 0.116 ± 0.2171 0.068 ± 0.1456
A versus B 6.69E-06 ± 8.3638E-06 ** 2.378E-06 ± 3.0695E-06**
A versus O 0.118 ± 0.2358 0.057 ± 0.1187
B versus O 0.097 ± 0.2122 0.050 ± 0.1050