Table 2 Primers used for intron amplification and sequencing.
Primers | Sequences | Position in PSA | References |
|---|---|---|---|
1400F | 5'-TGTACACACCGCCCGTC-3' | SSU rDNA, 1659 | Oliveira and Ragan [2] |
iF-1 | 5'-ACAAGGTTTCCGAAAGGG-3' | Intron, 1798 | Oliveira and Ragan [2] |
iR-3 | 5'-TTAATGTCGTGACCGCGCA-3' | Intron, 2306 | Oliveira and Ragan [2] |
iF-2 | 5'-AAGTCGCTTTTGTTGGC-3' | Intron, 2382 | Oliveira and Ragan [2] |
iR-2 | 5'-TTCGGACTGACTGCGTCG-3' | Intron, 2461 | Oliveira and Ragan [2] |
iF-3 | 5'-CGCTGGATGGTAATAAGGTG-3' | Intron, 2580 | Oliveira and Ragan [2] |
iR-1 | 5'-GACTCTGCTTTCGCAG-3' | Intron, 2730 | Oliveira and Ragan [2] |
18S3' | 5'-GATCCTTCTGCAGGTTCACCTACGGAA-3' | SSU rDNA, 2860 | Oliveira and Ragan [2] |
COX 2F | 5'- GTACCWTCTTTDRGRRKDAAATGTGATGC -3' | cox2-3, 1 | Zuccarello et al. [43] |
COX 3R | 5'- GGATCTACWAGATGRAAWGGATGTC -3' | cox2-3, 433 | Zuccarello et al. [43] |
F993 | 5'- GGTACTGTTGTAGGTAAATTWGAAGG -3' | rbcL - S, 1 | Freshwater et al. [46] |
RBCS3'R | 5'- GTTCTTGTGTTAATCTCAC -3' | rbcL - S, 571 | Freshwater et al. [46] |