Figure 1

Western blotting with SecY antisera of cyanelle membranes separated on SDS gels. Preadsorption, detection with affinity purified antisera which underwent preadsorption to the heptadekapeptide; TM, thylakoid membranes; IEM, inner envelope membranes. (A) Western blotting of thylakoid membranes (30 μg protein) with unpurified vs. affinity purified cyanelle SecY antisera. Thylakoid membranes were treated with sample buffer at 37°C for 30 min (37), 60°C for 10 min (60), 100°C for 3 min (100). (B)Western blotting of cyanelle membranes separated on SDS gels with affinity purified anti-cyanelle SecY. TM1 (20 μg protein) and IEM (30 μg protein) were treated with sample buffer at 37°C for 30 min, TM2 (20 μg protein) was treated at 100°C for 3 min.