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Figure 5 | BMC Evolutionary Biology

Figure 5

From: The promastigote surface antigen gene family of the Leishmania parasite: differential evolution by positive selection and recombination

Figure 5

Analysis of chimeric PSA genes by Simplot. A) LmjF12.0990. A DNA alignment of the indicated genes covering LRR #2, 9 and the last 4 C-ter most LRRs, together with the N-ter and CGC-C2 flanking domains (Additional file 14) was analysed with Simplot. Single or multiple genes from each phylogenetic PSA gene cluster of L. major Chr 12 (see Fig. 3) were first used for similarity analysis (Simplot) against the LmjF12.0990 putative chimeric gene. The six genes of cluster 12C were reduced to a consensus sequence. The best pair of candidate parental genes (LmjF12.0760 and consensus sequence for cluster 12C) and a control gene were then used for bootscanning and informative sites analysis (MaxChi2). B) LmjF12.0960. The same analysis was performed as in A) using a DNA alignment covering LRR #2, 9 and the last 3 C-ter most LRRs (see Additional file 15).

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