Figure 2

Annotated structure of human eRF1 alone and superimposed with yeast Dom34p structure. Panel A: Human eRF1 (PDB accession code 1DT9) structure is shown indicating the location of functional features and patches of residues that are highly conserved across the eRF1 family. Panel B: Superposition of human eRF1 (green) and S. cerevisae Dom34p (PDB accession code 2VGM, light pink). eRF1-specific functional motifs are marked with the same color coding as in panel A. Color code and residue coordinates (from figure 2B) are as follows: GGQ motif (yellow, 198–200), NIKS (blue, 62–65) and YXCXXF (orange, 138–144) motifs, GILRY motif (red, 441–445), a/eRF1-specific insertion (magenta, 356–393) RNA-binding motif [30] (white, 320–352), E41, E/D44 and D 45 residues in Dom34p (violet) and M stem conserved region (cyan, 239–245).