Figure 2

The IGS-ETS of Bacillus stick insects. Schematic drawing of the IGS-ETS structure of Bacillus stick insects. The IGS region is characterized by a large array of tandemly repeated head-to-tail sequences, here named Bag530, that are proposed to be RNA-PolI enhancers. Downstream the last Bag530 sequence, the transcription start point (tsp) mark the IGS-ETS boundary. Redundant tsp sequences are also found in each Bag530 enhancer. The ETS show a twofold 388 bp direct repeats (Bag388a and Bag388b), with an unknown regulatory function. Sequenced fragments are reported in gray: fragments obtained by PstI restriction analysis (PstI₅₃₀ and PstI₅₅₀₀) are marked with gray rhombs (◆); ^3'IGS-ETS fragment, obtained by PCR, is marked with gray circles (●).