The evolution of TEP1, an exceptionally polymorphic immunity gene in Anopheles gambiae

Table 2 Haplotype frequency tests

	n	S	θ	K	M	Haplotype configuration
Arabiensis^a	19	79	22.6	7*	7*	(4,1,0,0,0,1,1,0,0,0,0,0,0,0,0,0,0,..)***
TEP1s	9	10	3.7	5	3	(3,0,2,0,0,0,0,0,0,0,0,0,0,0,0,0,0,..)
TEP1r	12	8	2.6	4	6	(2,0,0,1,0,1,0,0,0,0,0,0,0,0,0,0,0,..)
Mbita	17	85	25.1	3***	9***	(0,0,0,2,0,0,0,0,1,0,0,0,0,0,0,0,0,..)***
TEP1s	24	10	2.7	4	16	(0,0,1,1,0,0,0,0,0,0,0,0,0,0,0,1,0,..)
TEP1r	10	2	0.7	2	6	(0,0,0,1,0,1,0,0,0,0,0,0,0,0,0,0,0,..)
Cameroon	24	20	5.4	4*	15*	(0,1,1,1,0,0,0,0,0,0,0,0,0,0,1,0,0,..)***
TEP1s	9	8	2.9	3	4	(0,1,1,1,0,0,0,0,0,0,0,0,0,0,0,0,0,..)
TEP1s/r	15	0	0.0	1^b	15^b	(0,0,0,0,0,0,0,0,0,0,0,0,0,0,1,0,0,..)^b

n is the number of haplotypes sequenced, S the number of segregating sites, θ Watterson's estimate of 4 Nμ per gene, K the number of haplotypes, M the frequency of the commonest haplotype. The 'Haplotype Configuration' is a vector (n₁, n₂, n₃, ..., n_i) giving the number of distinct haplotypes that appeared i times in the sample (see Innan et al 2005). For example, the first haplotype configuration (4,1,0,0,0,1,1,...) has four haplotypes that appeared once each, one that appear twice, none that appeared three, four or five times, one that appeared six times and one that appeared seven times. ^aSampling was not at random with respect to s/r state, ^bsignificance not tested. Significance assessed through coalescent simulation is indicated as *p < 0.05, ***p < 0.001.

ISSN: 2730-7182