Figure 4

Phylogenetic reconstruction of CHEK2 duplicon events in anthropoids. (A) Phylogenetic analysis of the CHEK2 duplicon family. A neighbor-joining phylogram was generated using MEGA 4.0 software [41] and rooted on the ancestral CHEK2 sequence of the rhesus macaque. We used 1951 bp of human and chimpanzee CHEK2 duplicons and ancestral CHEK2 sequences of human, chimpanzee and rhesus macaque. Ancestral loci are highlighted by a red box. Branch lengths are proportional to the number of nucleotide changes between taxa and are indicated below each respective branch (>0.001). Bootstrap values > 70 from 1000 replicates are indicated above each corresponding branch point. Red arrows indicate the LINE1 integration and the pericentromeric "seed" and onset of "swaps". (B) Corresponding FISH signals with fosmid WI2-1621D20 on chromosomes of human (HSA), chimpanzee (PTR), gorilla (GGO), orangutan (PPY), white-cheeked crested gibbon (NLE), rhesus macaque (MMU) and common marmoset (CJA). The left column represents all chromosomes, for which signals were unambiguously correlated to the respective sequences used for MEGA 4.0 analysis. Black arrows indicate the corresponding FISH signals on chromosomes 22. All other signal-containing chromosomes for which no corresponding sequence was used for MEGA4 analysis are arranged in the grey box. These chromosomes are ordered with respect to their human orthologous chromosomes. Chromosomes showing a signal for which no corresponding CHEK2 duplicon sequence was available are displayed on the top and are indicated by asterisks.