Table 4 Primer nucleotide sequence and expected amplicon size. Accession numbers in GeneBank for the identified sialyltransferases and β-actin sequences.
Primer | Sequence | Accession number | Product size (bp) |
|---|---|---|---|
ST8Sia I | Forward 5'-TTGCGGTTACTAAGGAGA Reverse 5'-ACGAAAGATTTGCGGGAC | AJ715535 | 346 |
ST8Sia II | Forward 5'-GACTCGCACGACTTTGTT Reverse 5'-TGGTTGGTCAGCCAGTAA | AY055462 | 335 |
ST8Sia III | Forward 5'-AACAACCTGCTGACCATCC Reverse 5'-ATGATACGGCAGCTCCTT | AJ715543 | 354 |
ST8Sia IV | Forward 5'-TCTTGACTTGGGAGTTGG Reverse 5'-TCTGACCGCAATCCTACA | AJ715545 | 366 |
ST8Sia V | Forward 5'-AAATAAGGAGGAGACGGATAA Reverse 5'-AAAGTCAGAAGCGTCAAT | AJ715546 | 291 |
ST8Sia VI | Forward 5'-TGTCTATGATGGCGAAAG Reverse 5'-TGACCGTATGAATGAAGG | AJ715551 | 333 |
ST8Sia VIIA | Forward 5'-TTTCCTGGTGGTCCTGAT Reverse 5'-GGTGCGTCTACTGTTGGTT | AM287257 | 346 |
β-actin | Forward 5'-GTTGGTATGGGACAGAAAGA Reverse 5'-GGCGTAACCCTCGTAGAT | AF025305 | 378 |