Expression of Nv-Runx and Nv-CBFβ in the head region of adult Nematostella anemones. A single anemone specimen was cut into four parts, as shown in the diagram: tentacles, head/pharyngeal region (including tentacle bases), body column, and physa. Reverse-transcription (RT) PCR was performed on an equal amount of total RNA extracted from each of the four body regions. An equivalent fraction of the amplification reaction was visualized on an agarose gel. Using this assay, Nv-Runx expression was detected in the tentacles, and to a lesser extent in the head/pharyngeal region, but not in the column or physa. As a positive control, actin was found to be expressed at high levels in all four body regions.