Figure 6
Limited proteolysis of lampER with E 2 and of amphiER with various ER ligands. Human ERα was used as a positive control. lane 1: undigested protein, lanes 2–5, 6–9: digested protein in the absence (lane 2 and 6) or presence (lanes 3–5 and 7–9) of ligand (10-3M to 10-5M). 2 different trypsine doses are shown, indicated by thick or thin bars above each panel. The ligands are (A) estradiol, (B) 3β-Androstane-diol, (C) 4-hydroxytamoxifen, (D) diethylstibestrol, (E) enterolactone, (F)ICI-182780 and (G) bisphenol A.