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Table 1 Chlorophyte species used for this investigation.

From: ITS2 data corroborate a monophyletic chlorophycean DO-group (Sphaeropleales)

Clade Species Strain GenBank
'Sphaeroplea ' Ankyra judayi (G.M. Smith) Fott 1957 SAG 17.84 EU352800
  Atractomorpha porcata Hoffman 1984 strain SAG 71.90 EU352803
  Sphaeroplea annulina (Roth) C. Agardh 1824 SAG 377.1a EU352801
  Sphaeroplea annulina (Roth) C. Agardh 1824 SAG 377.1e EU352802
'Dunaliella' Haematococcus droebakensis Wollenweber 1908 - U66981
  Dunaliella parva Lerche 1937 - DQ116746
  Dunaliella salina (Dunal) Teodoresco 1905 CCAP 19/18 EF473746
'Hydrodictyon ' Hydrodictyon africanum Yamanouchi 1913 UTEX 782 AY779861
  Hydrodictyon patenaeforme Pocock CCAP 236/3 AY577736
  Hydrodictyon reticulatum (Linnaeus) B. de St.-Vincent 1824 CBS AY779862
  Pediastrum braunii Wartmann 1862 SAG 43.85 AY577756
  Pediastrum duplex Meyen 1829 UTEX 1364 AY779868
  Pseudopediastrum boryanum (Raciborski) Sulek 1969 UTEX 470 AY779866
  Sorastrum spinulosum Nägeli 1849 UTEX 2452 AY779872
  Stauridium tetras (Ehrenberg) Ralfs 1844 EL 0207 CT AY577762
'Oedogonium' Bulbochaete hiloensis (Nordstedt) Tiffany 1937 - AY962677
  Oedogonium cardiacum (Hassall) Wittrock 1870 - AY962675
  Oedogonium nodulosum Wittrock 1872 - DQ078301
  Oedogonium oblongum Wittrock 1872 - AY962681
  Oedogonium undulatum (Brébisson) A. Braun 1854 - DQ178025
'Reinhardtii' Chlamydomonas incerta Pascher 1927 SAG 81.72 AJ749625
  Chlamydomonas komma Skuja 1934 - U66951
  Chlamydomonas petasus Ettl SAG 11.45 AJ749615
  Chlamydomonas reinhardtii Dangeard 1888 CC-620 AJ749638
  Chlamydomonas typica Deason & Bold 1960 SAG 61.72 AJ749622
  Eudorina elegans Ehrenberg 1831 ASW 107 AF486524
  Eudorina unicocca G.M. Smith 1930 UTEX 1215 AF486525
  Gonium octonarium Pocock 1955 Tex AF054424
  Gonium pectorale O.F. Müller 1773 Chile K AF054440
  Gonium quadratum E. G. Pringsheim ex H. Nozaki Cal 3-3 AF182430
  Pandorina morum (O.F. Müller) Bory de Saint-Vincent 1824 Chile AF376737
  Volvox dissipatrix (Shaw) Printz - U67020
  Volvox rousseletii G.S.West - U67025
  Volvulina steinii Playfair 1915 - U67034
  Yamagishiella unicocca (Rayburn & Starr) Nozaki 1992 ASW 05129 AF098181
'Scenedesmus' Desmodesmus abundans (Kirchner) Hegewald 2000 UTEX 1358 AJ400494
  Desmodesmus bicellularis (Chodat) An, Friedl & Heg. 1999 CCAP 276/14 AJ400498
  Desmodesmus communis (Hegewald) Hegewald 2000 UTEX 76 AM410660
  Desmodesmus elegans (Hortobágyi) Heg. & Van. 2007 Heg 1976–28 AM228908
  Desmodesmus opoliensis (P.G. Richter) Hegewald 2000 EH 10 AM410655
  Desmodesmus pleiomorphus (Hindák) Hegewald 2000 UTEX 1591 AM410659
  Desmodesmus quadricauda (Turpin) Hegewald - AJ400495
  Scenedesmus acuminatus (Lagerheim) Chodat 1902 UTEX 415 AJ249511
  Scenedesmus acutiformis (B. Schröder) F. Hindák 1990 SAG 276.12 AJ237953
  Scenedesmus basiliensis Chodat 1926 UTEX 79 AJ400489
  Scenedesmus dimorphus (Turpin) Kützing 1833 UTEX 417 AJ400488
  Scenedesmus longus Meyen 1829 ex Ralfs NIOO-MV5 AJ400506
  Scenedesmus obliquus (Turpin) Kützing 1833 Tow 9/21P-1W DQ417568
  Scenedesmus pectinatus Meyen 1828 An 111a AJ237954
  Scenedesmus platydiscus (G.M. Smith) Chodat 1926 UTEX 2457 AJ400491
  Scenedesmus raciborskii Woloszynska 1914 An 1996–5 AJ237952
  Scenedesmus regularis Svirenko Heg 1998–2 AY170857
  Scenedesmus wisconsinensis (G.M. Smith) Chodat 1996 An 41 AJ237950
  1. Listed is the current clade classification of the species [69, 70, 24] and the GenBank accession numbers of the analyzed sequences. The four newly obtained sequences are of the 'Sphaeroplea' clade.