The molecular basis of color vision in colorful fish: Four Long Wave-Sensitive (LWS) opsins in guppies (Poecilia reticulata) are defined by amino acid substitutions at key functional sites

Table 3 Standard curve log fit values for quantitative PCR.

(Plasmid) Standard Curve	Linear Equation	Efficiency (%)	RSq
A180	Y = -3.315(logX) + 37.05	100.3	0.999
S180	Y = -2.992(logX) + 35.77	115.9	0.996
S180r	Y = -3.670(logX) + 38.96	87.3	0.985
P180	Y = -3.319(logX) + 36.68	100.1	0.999

The linear equation, efficiency and r-squared (RSq) values for the qPCR standard curve are shown. Plasmid copy numbers used as standards were determined by calculating the number of moles in each sample multiplied by Avogadro's number. A standard curve of Ct (dR) was used to calculate original copy number of mRNA transcripts in the diluted cDNA samples.

ISSN: 2730-7182