The H orthologue of Apis mellifera. A) Schematic of the A.m.H gene. Location of SBD, GBD and CBD is shown. The dashed line below represents the fragment used to screen the Apis cDNA library and to generate fusion-protein for antisera production, respectively. Relevant restriction sites are indicated above the map. ERI = Eco RI, ERII = Eco RII, BHI = Bam HI, XI = Xho I. B) pI composition of the H orthologues. The red arrow indicates the acidic domain inside the SBD. Asterisk: acidic domain absent from A.m.H. C) Alignment of the orthologous H proteins from D. melanogaster (above, D.m.H) and A. mellifera (below, A.m.H). Start codons are highlighted in cyan; in D.m.H the second is the major cap-dependent start site, whereas the third is the internal ribosome entry site . The alignment above shows the start region if the first methionine is ignored. SBD, GBD and CBD are underlined. Nuclear localization consensus sequences (NLS) are boxed in green. Black arrows: position of introns. Blue: identical residues, red: highly similar residues, yellow: similar residues. Black dots represent gaps.