Distribution of 97 sampling sites and geographic distribution of L-G1C12 haplotypes in unisexuals and Ambystoma laterale in northeastern North America (see Additional file 1 for description of locations and sample sizes). Numbers represent sampling sites where we collected specimens for this study. The shaded grey area shows the current known range of unisexual Ambystoma. Small map on the top corresponds to the boxed area on the large map. A-F represent six L-G1C12 haplotypes. Blue and green letters indicate populations where we only sampled unisexuals or A. laterale, respectively. Red letters indicate populations where both unisexual and A. laterale were found and they shared the same haplotypes. Multiple letters divided by slashes represent populations where more than one haplotype was found.