Table 2 Polymorphic markers differentiating N2 and DR1350

I

1

pkP1003

G/A

126950

gtatcctcatccttctaccacc

gcgtcgttccacgtgttatgc

Rsa I

2

pkP1098

C/T

360847

tatcatgctggcgtagatttc

tggataaaaagcgtttctgg

Hpa II

3

pkP1051

A/T

825028

cctacaacaggcaaagaagc

aattcctaccaaagctccgc

Ssp I

4

pkP1102

C/A

1517890

tggaaggatattgtggcg

ctgaacgcgattctcctgtg

Hinf I

5

pkP1016

T/G

1882334

gacaatgaccaataagacg

gatccgtgaaattgttccg

Bsr I

6

snp_F28H1[1]

C/T

3989631

tgccaaaatagcagtaggc

tgaaactgcaataacataacg

Hpy CH4V

II

1

W08F4/33109

T/C

593962

cagacttccaccgtaccattg

gagacgaaacgatttacgagg

Dra I

2

pkP2134

C/T

862482

atcaggatccggaacagtcg

tcgtaaatggtcagttttgg

Dde I

3

pkP2010

T/C

1129821

taatttctagcaccagtgaggc

cccaaatttccacctgtaatcc

Dde I

4

pkP2015

A/G

1640462

gtacctaccgtcattgatagtg

ctttcagtggacagaatccg

Pvu II

5

pkP2136

C/T

1683953

agttgtgttatacttgttgg

tgtctaactgaagagatgacg

Xba I

6

T05A8/28596

G/A

2631509

ctatggtgcatcgaagtgtc

gtcagcacgttcttaaccttg

Bam HI

7

pkP2026

T/C

2737466

cgatggattatgtggtgagtc

caggttggtcatcatttcagac

Sty I

8

pkP2114

C/G

2755074

tcacgtcgtcacctacgcc

aatctgaccaaggtatcgg

Alu I

9

nP133

-/TTCCG

2769667–8

aacgttcaaaagtgataggtc

ttctcactcggtgtactcgg

Eco RI

10*

snp_Y25C1A[1]

C/A

3077209

accgtctttcagcgctcgacg

caaaattctgctgataatgg

HpyCH 4V

11

F19B10/12159

G/T

3660181

ctgcttctctgcaagtctgc

cttggaacagtctctcaacg

Dra II

12*

snp_C49D10[3]

A/T

3868386

agaacatctatcacgacttgg

tttgtgtcatatttgcgtcc

Tsp509 I

13

pkP2051

G/A

4299265

gcgtgttttttccgtcgatcg

cagcgtccagactggtttgg

Hae II

14*

snp_B0304[5]

GG/TT

4526832–3

acatcaccaattacacgacc

agatcgtacttattgtagcc

Eco 0109I

15

C18A3/8661

G/A

5710053

catgtggacgacgtgtactgg

caatgtgcagtcgtctactgg

Pvu I

16

K10B2/5930

C/T

6362593

ccttgtactcgggaacacgc

gaatcagtcaaacgctgcgg

Sfu I

17

pkP2109

C/T

9401077

tgaacccataacagcttctgc

aactcgtgcgctctccttgg

Eco RI

18

pkP2069

G/T

10489659

tcaaccttcatacgtgtcgc

ggaatgactgataaaggtgtcg

Xba I

19

snp_W01G7[1]

G/C

14044928

tatataggtacctaaagagc

atttttgtccccttatatgg

Cac 8I

III

1

pkP3002

A/T

743326

ctgcttatagtcttcctgtcg

gcaaccccaccttcaatgac

Ssp I

2

snp_Y46E12[4]

T/C

1765120

tctaatgttttttccaatcagc

tatgattttactgctgctgg

Ssp I

3

pkP3099

T/C

5625446

tctttcagtgggctaacacc

tgcgtgggcagcccaaatacg

Hpy CH4V

4

B0361/15143

A/C

7279588

gtatttcttacccgagagtcc

cagttcacctggaatctcaatc

Dde I

5

snp_C50C3[2]

G/A

8180784

gctcttcttggtacgttccc

cgcgtcttctgagtgtttcc

Alu I

6

snp_T05G5[1]

T/A

9742847

cgtaaactaccaaactcggtg

ggtctactacaactatacaggc

Dra I

7

pkP3059

A/G

10613168

actcggccacgtggcaagc

aaagcctttcggaacttcc

Xba I

8

snp_F56A8[1]

C/T

13247630

tttggaggaccatcagagg

tggctcaccttctttctcc

Sau 3A

X

1

snp_T23F2[1]

T/A

5492084

tttccggcagatgcaccacg

tgcaaatagctgatcactgg

Dde I

2¹

C03B1/38853–6

TATA/CG

6374380–3

cgtagagacgcaaaataggc

gctcaatttcacgcgtccag

Acc I

3¹

C25B8/5416

C/G

6374374

gtatctgagatagggtgcgc

cggaaaacctgttagacatgg

Xba I

4¹

F41C6/10186

A/G

6879298

gtttggtcgctggagttttgg

catcaaagaggcaacaagggg

Fsi I

5²

pkP6158

C/T

8691678

aagacacccatccatgcatatc

caattgtcagccgttgtttc

Hind III

6²

pkP6030

T/G

8814093

gtaatcggttactgtgcactg

ctacatcaatgtcaacaccagc

Dde I

7

pkP6137

C/T

9677614

gccttggagagtctcgatttg

ttctgaccaccatagccgaac

Hinf I

8

pkP6039

T/C

10652947

cctcatctcatctttgcttg

caagatgacttgccgattcatg

Mse I

9

snp_F23D12[1]

T/C

14429597

taaacagaaaaattcacaaa t/c

atatttgaatggagtttcacc

†

10

pkP6167

C/T

15106709

taatagccgccaaagtgcg

gtgaagcaagtttgattttcc

Hinf I

11

snp_C02C6[7]

A/G

15561287

ttcgcgcatttatcttgtcc

tatattcattgatctaagtgc

Hpy CH4V

12

pkP6096

A/T

16389174

gattgaacatagctcacagc

tttcgatcgttttggacgcc

Rsa I