| |
Name
|
N2/DR1350
|
Location
|
Forward
|
Reverse
|
Enzyme
|
---|
I
|
1
|
pkP1003
|
G/A
|
126950
|
gtatcctcatccttctaccacc
|
gcgtcgttccacgtgttatgc
|
Rsa I
|
|
2
|
pkP1098
|
C/T
|
360847
|
tatcatgctggcgtagatttc
|
tggataaaaagcgtttctgg
|
Hpa II
|
|
3
|
pkP1051
|
A/T
|
825028
|
cctacaacaggcaaagaagc
|
aattcctaccaaagctccgc
|
Ssp I
|
|
4
|
pkP1102
|
C/A
|
1517890
|
tggaaggatattgtggcg
|
ctgaacgcgattctcctgtg
|
Hinf I
|
|
5
|
pkP1016
|
T/G
|
1882334
|
gacaatgaccaataagacg
|
gatccgtgaaattgttccg
|
Bsr I
|
|
6
|
snp_F28H1[1]
|
C/T
|
3989631
|
tgccaaaatagcagtaggc
|
tgaaactgcaataacataacg
|
Hpy CH4V
|
II
|
1
|
W08F4/33109
|
T/C
|
593962
|
cagacttccaccgtaccattg
|
gagacgaaacgatttacgagg
|
Dra I
|
|
2
|
pkP2134
|
C/T
|
862482
|
atcaggatccggaacagtcg
|
tcgtaaatggtcagttttgg
|
Dde I
|
|
3
|
pkP2010
|
T/C
|
1129821
|
taatttctagcaccagtgaggc
|
cccaaatttccacctgtaatcc
|
Dde I
|
|
4
|
pkP2015
|
A/G
|
1640462
|
gtacctaccgtcattgatagtg
|
ctttcagtggacagaatccg
|
Pvu II
|
|
5
|
pkP2136
|
C/T
|
1683953
|
agttgtgttatacttgttgg
|
tgtctaactgaagagatgacg
|
Xba I
|
|
6
|
T05A8/28596
|
G/A
|
2631509
|
ctatggtgcatcgaagtgtc
|
gtcagcacgttcttaaccttg
|
Bam HI
|
|
7
|
pkP2026
|
T/C
|
2737466
|
cgatggattatgtggtgagtc
|
caggttggtcatcatttcagac
|
Sty I
|
|
8
|
pkP2114
|
C/G
|
2755074
|
tcacgtcgtcacctacgcc
|
aatctgaccaaggtatcgg
|
Alu I
|
|
9
|
nP133
|
-/TTCCG
|
2769667–8
|
aacgttcaaaagtgataggtc
|
ttctcactcggtgtactcgg
|
Eco RI
|
|
10*
|
snp_Y25C1A[1]
|
C/A
|
3077209
|
accgtctttcagcgctcgacg
|
caaaattctgctgataatgg
|
HpyCH 4V
|
|
11
|
F19B10/12159
|
G/T
|
3660181
|
ctgcttctctgcaagtctgc
|
cttggaacagtctctcaacg
|
Dra II
|
|
12*
|
snp_C49D10[3]
|
A/T
|
3868386
|
agaacatctatcacgacttgg
|
tttgtgtcatatttgcgtcc
|
Tsp509 I
|
|
13
|
pkP2051
|
G/A
|
4299265
|
gcgtgttttttccgtcgatcg
|
cagcgtccagactggtttgg
|
Hae II
|
|
14*
|
snp_B0304[5]
|
GG/TT
|
4526832–3
|
acatcaccaattacacgacc
|
agatcgtacttattgtagcc
|
Eco 0109I
|
|
15
|
C18A3/8661
|
G/A
|
5710053
|
catgtggacgacgtgtactgg
|
caatgtgcagtcgtctactgg
|
Pvu I
|
|
16
|
K10B2/5930
|
C/T
|
6362593
|
ccttgtactcgggaacacgc
|
gaatcagtcaaacgctgcgg
|
Sfu I
|
|
17
|
pkP2109
|
C/T
|
9401077
|
tgaacccataacagcttctgc
|
aactcgtgcgctctccttgg
|
Eco RI
|
|
18
|
pkP2069
|
G/T
|
10489659
|
tcaaccttcatacgtgtcgc
|
ggaatgactgataaaggtgtcg
|
Xba I
|
|
19
|
snp_W01G7[1]
|
G/C
|
14044928
|
tatataggtacctaaagagc
|
atttttgtccccttatatgg
|
Cac 8I
|
III
|
1
|
pkP3002
|
A/T
|
743326
|
ctgcttatagtcttcctgtcg
|
gcaaccccaccttcaatgac
|
Ssp I
|
|
2
|
snp_Y46E12[4]
|
T/C
|
1765120
|
tctaatgttttttccaatcagc
|
tatgattttactgctgctgg
|
Ssp I
|
|
3
|
pkP3099
|
T/C
|
5625446
|
tctttcagtgggctaacacc
|
tgcgtgggcagcccaaatacg
|
Hpy CH4V
|
|
4
|
B0361/15143
|
A/C
|
7279588
|
gtatttcttacccgagagtcc
|
cagttcacctggaatctcaatc
|
Dde I
|
|
5
|
snp_C50C3[2]
|
G/A
|
8180784
|
gctcttcttggtacgttccc
|
cgcgtcttctgagtgtttcc
|
Alu I
|
|
6
|
snp_T05G5[1]
|
T/A
|
9742847
|
cgtaaactaccaaactcggtg
|
ggtctactacaactatacaggc
|
Dra I
|
|
7
|
pkP3059
|
A/G
|
10613168
|
actcggccacgtggcaagc
|
aaagcctttcggaacttcc
|
Xba I
|
|
8
|
snp_F56A8[1]
|
C/T
|
13247630
|
tttggaggaccatcagagg
|
tggctcaccttctttctcc
|
Sau 3A
|
X
|
1
|
snp_T23F2[1]
|
T/A
|
5492084
|
tttccggcagatgcaccacg
|
tgcaaatagctgatcactgg
|
Dde I
|
|
21
|
C03B1/38853–6
|
TATA/CG
|
6374380–3
|
cgtagagacgcaaaataggc
|
gctcaatttcacgcgtccag
|
Acc I
|
|
31
|
C25B8/5416
|
C/G
|
6374374
|
gtatctgagatagggtgcgc
|
cggaaaacctgttagacatgg
|
Xba I
|
|
41
|
F41C6/10186
|
A/G
|
6879298
|
gtttggtcgctggagttttgg
|
catcaaagaggcaacaagggg
|
Fsi I
|
|
52
|
pkP6158
|
C/T
|
8691678
|
aagacacccatccatgcatatc
|
caattgtcagccgttgtttc
|
Hind III
|
|
62
|
pkP6030
|
T/G
|
8814093
|
gtaatcggttactgtgcactg
|
ctacatcaatgtcaacaccagc
|
Dde I
|
|
7
|
pkP6137
|
C/T
|
9677614
|
gccttggagagtctcgatttg
|
ttctgaccaccatagccgaac
|
Hinf I
|
|
8
|
pkP6039
|
T/C
|
10652947
|
cctcatctcatctttgcttg
|
caagatgacttgccgattcatg
|
Mse I
|
|
9
|
snp_F23D12[1]
|
T/C
|
14429597
|
taaacagaaaaattcacaaa t/c
|
atatttgaatggagtttcacc
|
†
|
|
10
|
pkP6167
|
C/T
|
15106709
|
taatagccgccaaagtgcg
|
gtgaagcaagtttgattttcc
|
Hinf I
|
|
11
|
snp_C02C6[7]
|
A/G
|
15561287
|
ttcgcgcatttatcttgtcc
|
tatattcattgatctaagtgc
|
Hpy CH4V
|
|
12
|
pkP6096
|
A/T
|
16389174
|
gattgaacatagctcacagc
|
tttcgatcgttttggacgcc
|
Rsa I
|
- For chromosomes I, II, III and X, for each polymorphism, its name, the N2/DR1350 polymorphism, its physical location in N2 [23], the forward and reverse PCR oligonucleotide primers (5'-3') and, where appropriate, the restriction enzyme used for genotyping. * denotes markers only used in the analysis of the NILs. 1 and 2 denote two groups of markers that could not be separated genetically. † denotes the marker scored in the RILs by allele specific PCR.