Figure 10

Annotated tree of the CFTR 3'UTR regulatory region. The NCBI common species tree [28,29] labeled with TFBS predictions for Oct4, Sox2, and HOMF only, for the region shown in Figure 9. The boxes contain TFBS predictions for each node in the tree. We use the TFBS names of Mapper and Genomatix. According to Dollo parsimony the TFBSs written in green are gained at the corresponding node, the red ones are lost. In blue we list TFBSs that are estimated to be present for the (ancestral) species at the node in question. We infer that Oct 114 (the Oct binding site at position 114) and Sox 126 were already present for all amniotes, and that they both disappeared for platypus (Ornithorhynchus anatinus) and mouse. Another come-and-go pattern can be seen in case of Oct 86 and Sox2 167: We infer that both have evolved in the lineage from amniotes to mammals, and disappeared in the lineage leading to the marsupial (Macropus).