Figure 5

Distribution of CR1B and CR1A families in Lepidoptera based on PCR amplification. Phylogeny of studied superfamilies and families of butterfly suborder Ditrysia according to [34], with modifications (A). Families within Papilionoidea and Bombycoidea are shown since these superfamilies were analyzed in more detail. (B) PCR amplification of lepidopteran species with the primer pairs CR1-S/CR1-A (expected amplicon length 550 bp), CR1A-S/CR1A-A (expected amplicon length 520 bp) and CR1B-S/CR1B-A (expected amplicon length 520 bp) resolved in 1.3% agarose gel and stained with ethidium bromide. Lane "-", negative control; lane M, 100 bp DNA ladder.