Alternative splicing of A. gambiae GMCβ4 gene. The figure is shown in a conventional direction of 5' to 3' from left to right (opposite to the direction in Figure 2). The length of exons and introns are shown in base pairs. Exon a (in gray color) contains the highly conserved ADP-binding domain (arrow) and is predicted as a shared exon in all isoforms. Exons b-e are predicted to be alternatively spliced C-terminal exons. The splicing patterns of isoforms indicated in the phylogeny (Figure 3) are as follows: "iso1", exons a/b; "iso2", exons a/c, "iso3", exons a/d; "iso4", exons a/e. The detailed sequence information of this gene can be found in Additional File 5.