Skip to main content

Table 4 Primers used for PCR, RACE, and genome-walking to generate rpoD and tsg1 gene sequences.

From: Chloroplast His-to-Asp signal transduction: a potential mechanism for plastid gene regulation in Heterosigma akashiwo (Raphidophyceae)

Primer name Sequence
PCR  
M13 forward 5'-GTAAAACGACGGCCAG-3'
M13 reverse 5'-CAGGAAACAGCTATGAC-3'
ORAC 1104 5'-AGGCTGACGAAGCTTGTGCAA-3'
ORAC 1105 5'-TTCTCTACCTACGCAACATGGTGG-3'
RACE  
ORAC 240 5'-GATACGGTGAAGGACAAGGTCTCATC-3'
ORAC 242 5'-CAGTGGCTTCTGCAAGCTCCGCGAG-3'
Genome walking  
ORAC 231 5'-TACCCAAAGTCTTCTCCAGTGTAACTAGAG-3'
ORAC 230 5'-GAATTTCTTCTTGTGCCGTCAACATATCG-3'