Figure 2From: Evolutionary proteomics identifies amino acids essential for ligand-binding of the cytokinin receptor CHASE domainThe CHASE domain of CRE1/AHK4 is necessary and sufficient for cytokinin binding. In vitro binding of trans-[2-3H]zeatin to full length CRE1/AHK4 protein or different domains of the receptor overexpressed in E. coli BL21. Bacterial cells were assayed for specific trans-[2-3H]zeatin binding. Data are mean ± s.d.; n = 4.Back to article page