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Figure 5 | BMC Evolutionary Biology

Figure 5

From: Evolutionary genomic remodelling of the human 4q subtelomere (4q35.2)

Figure 5

FRG1 and FRG2 histone acetylation and transcription in African apes and humans. A) Chromatin immunoprecipitation experiments on gorilla and chimpanzee lymphoblastoid cell lines before (GGO and PTR) and after Trichostatin A treatment (GGO-TSA and PTR-TSA). Chromatin was immunoprecipitated with a rabbit antiserum anti-acetyl histone H4, and a normal rabbit IgG as pre-immune (Upstate Biotechnology); the FRG2 and FRG1 promoter regions were amplified on Input DNA, pre-immune and H4Ac-immunoprecipitated samples using the primer pairs shown in Additional file 5. B) Quantification by real-time RT-PCR of the mRNA of the FRG2 and FRG1 genes; total RNA from untreated (control) and TSA-treated (TSA) GGO, PTR and HSA fibroblast cell lines was used as a template with primer pairs for FRG1 and FRG2 mRNAs. Each experiment was performed in triplicate (bars show SD).

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