Figure 5

Alignment of representative porin sequences. Amino acid sequences from a plant (O. sativa VDAC1, Osat1,CAB82853), an animal (H. sapiens VDAC1, Hsap1, NP_003365), a fungus (N. crassa, Ncra, XP_323644) and a stramenopile (P. sojae, Psoj, C_1010023) were aligned using PRALINE, as described in Methods. Residues in grey background are predicted to reside in β-strands, overlined sequences in α-helical regions, and identical (|) and conserved (:) residues between adjacent pairs of sequences are indicated. Chemically-similar residues in all four representative sequences are shown in bold. The GLK motif (residues 87–89 of Osat1) and the existing eukaryotic porin motif (PS00558, residues 216–238 of Osat1) are indicated by dotted underlining. The VKAKV sequence (residues 224–228 of Osat1) is part of PS00558. Note that this motif is not found in the P. sojae VDAC sequence. Motifs, derived in this work, that are characteristic for plants (7–48 of Osat1), animals (4–18 of Hsap1) and fungi (1–16 and 68–74, separated by any 48–52 residues, Ncra) are underlined. A unique motif for the stramenopiles could not be discerned from the data available.