Figure 2

Neighbor-joining (NJ) and maximum likelihood (ML) phylogenetic reconstruction of the LLTP superfamily. Tree topology was based on the alignment of the conserved amino acid sequence motifs of the LLT module [see Additional file 2]. Horizontal lengths of branches are proportional to the estimated numbers of amino acid substitutions produced by the NJ algorithm. Numerals at each node show local bootstrap confidence levels estimated by bootstrap pseudoreplicates in NJ and ML, respectively. Numbers are indicated when at least one bootstrap confidence level value at each node is ≥75. The ML inference yielded a similar topology as the NJ tree at the level of significance used. However (-) indicates that this cluster was not formed in the ML analysis. Crustacean sequences are shaded in gray. Penaeus monodon apoCr and CP and Apis melliphera apoLp-II/I and Vtg are framed as an indication of the presence in one species of homologous extracellular proteins from two different LLTP families. A number in parentheses, corresponding to the extension number used in sequence databases, is attached to the species name, when more than one characterized protein was found in a defined LLTP family. The number in bracket after the species name refers to the code number used for species designation in Figure 3. Abbreviations of LLTP families and subfamilies are as in the legend to Figure 1.