Figure 4

Maximum Likelihood phylogenetic tree produced using sequences of NR ligand binding domain. Phylogenetic relationship was examined by the Maximum Likelihood (ML) method as described for Fig. 3. Support values for the tree were obtained by bootstrapping a 100 replicates and are indicated above each branch. Branches under the threshold value of 46 (this value was set to support the subfamily IV as a monophyletic group) were shown as polytomies. Bayesian inference with the same methods as in Fig. 3, by running 3 million generations. The PPs are shown below each branch or after the ML bootstrapping value separated by a slash. Star indicates the node obtained form by Bayesian inference which was different from that obtained by the ML method. The accession number of each sequence used for phylogenetic analysis can be found in additiona1 file 2 and 3.