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Figure 5 | BMC Evolutionary Biology

Figure 5

From: The invariant phenylalanine of precursor proteins discloses the importance of Omp85 for protein translocation into cyanelles

Figure 5

Binding of pFNR and mutants to proteoliposomes. pFNR (A), pFNR F→Y (B) and pFNR F→G (C), pFNR ΔF (D), or pFNR ΔAFV (E) were incubated with indicated concentrations of liposomes (black circles) or proteoliposomes (open circles) with a ratio of 1:2375 (protein to lipid, molar ratio). The binding was quantified as described and is expressed as percent of input of precursor protein. The data were fitted to equation 1. F) The ratio between the apparent dissociation constant determined for the binding to liposomes and for the binding to proteoliposomes is shown. The labelling of the x-axis is according to the sub-figure labelling.

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