Figure 2

Replacement of the aromatic amino acid changes lipid binding properties of pFNR. The binding of pFNR (A), pFNR F→Y (B) and pFNR F→G (C) to indicated amounts of immobilized PC (black circle), DGDG (open square), PG (grey triangle), 85 mol% DGDG/15 mol% PC (grey diamond) or 85 mol% PG/15 mol% PC (open grey hexagon) was determined as described and binding normalized to the input is shown in percent. In A-C) the average of at least three independent experiments and the standard deviation is shown. Lines represent the least square fit analysis to equation 1. (D) The apparent dissociation constant for the association of pFNR F→Y (black) and pFNR F→G (grey) with the indicated lipids was calculated (E1) and normalized to the apparent dissociation constant of the wild type protein for the according lipid. The dual logarithm is shown.